Title1: Label-free imaging of live cell morphology by path-length stabilized quantitative phase microscope
Abstract: Cell morphology and its dynamics are widely studied intrinsic properties of live cells. For example, the motility of cancer cells correlates with their metastatic properties, and the membrane fluctuations of red blood cells correlate with their membrane stiffness. In order to quantitatively visualize the dynamic morphology of live cells, we have developed a compact microscope named “Low-Coherent Quantitative Phase Microscope” (LC-QPM), which has the active stabilization of the path-length disturbance and works without optical tables.
Quantitative phase microscopes, also known as digital holography microscopes, have been applied to cell biology because optical thickness of live cells can be obtained non-invasively. Among the QPMs, our compact setup with low-coherent illumination is able to obtain full-field distribution of optical thickness without speckle noise or halo. Our microscope resolves the phase of the light-field and is able to visualize the membrane morphology in nanometer resolution.In this presentation, we show noiseless quantitative phase images and time-lapse movies obtained with live cell culture. The cells were kept intact during the image acquisition and showed their membrane fluctuations in nanometer resolution. Moreover, because our setup has an option to add the fluorescence imaging capability, some of our results come with an additional labeled fluorescence information.
汇报人： Toyohiko Yamauchi , Senior Researcher, the 7th Research Group Central Research Laboratory, Hamamatsu Photonics K.K.